Journal: Cells

Article Title: Proportions of Basement Membrane Proteins in Cerebrovascular Smooth Muscle Cells After Exposure to Hypercapnia and Amyloid Beta.

doi: 10.3390/cells14080614

Figure Lengend Snippet: Figure 4. BM protein expression in HBVSMCs cultured under normocapnia or hypercapnia, in the absence or presence of 100 nM Aβ40 or Aβ42. (a) Collagen IV expression was significantly increased under hypercapnia conditions. The presence of Aβ40 or Aβ42 did not significantly affect collagen IV levels. (b) Laminin expression was significantly decreased under hypercapnia conditions. The presence of Aβ40 or Aβ42 did not significantly affect laminin levels. (c) Fibronectin expression was significantly increased under hypercapnia conditions. The presence of Aβ40 or Aβ42 did not significantly affect collagen IV levels. (d) Perlecan expression was significantly increased under hypercapnia conditions relative to normocapnia but only in the presence of Aβ40 or Aβ42. HBVSMCs cultured under hypercapnia but in the absence of Aβ showed no change in collagen expression relative to normocapnia. Error bars represent standard deviation. p < 0.05 (*), p < 0.01 (**). Black dots represent biological replicates (n = 3).

Article Snippet: The BSA was removed and cells were incubated with antibodies against collagen IV (ab6586, Abcam, Cambridge, UK 1:400), laminin (L9393, Sigma, Burlington, MA, USA 1:200), fibronectin (F3648, Sigma, Burlington, MA, USA, 1:400), or perlecan (sc-25848, Santa Cruz, Dallas, TX, USA, 1:400).

Techniques: Expressing, Cell Culture, Standard Deviation

Journal: iScience

Article Title: Extracellular matrix protein composition dynamically changes during murine forelimb development

doi: 10.1016/j.isci.2024.108838

Figure Lengend Snippet: The ECM is differentially distributed within musculoskeletal tissues during forelimb development (A–R) Cryosections from E11.5-E14.5, P3 and adult forelimbs were stained with antibodies against: (A–F, A′–D′) type I collagen (COLI; green), type V collagen (COLV; red), and myosin heavy chain, a marker for differentiated skeletal muscle (MY32; blue); (G–L, G′–J′) tenascin-C (TNC; green), fibrillin-2 (FBN2; red), and MY32 (blue); (M–R, M′–P′) elastin microfibril interfacer-1 (EMILIN1; green), perlecan (HSPG2; red), and nidogen-2 (NID2; blue). Insets (indicated with ’) are a 3× enlargement of the region containing the nascent elbow (∗) for E11.5-E14.5. Scale bars represent 200 μm. Individual channels and secondary antibody only negative control panels are shown in Figures S3–S5 . (S) Graphical summary of protein dynamics.

Article Snippet: Rat monoclonal perlecan (HSPG2; clone A7L6) , Santa Cruz Biotechnology , Cat# sc-33707; RRID: AB_627714.

Techniques: Staining, Marker, Negative Control

Journal: iScience

Article Title: Extracellular matrix protein composition dynamically changes during murine forelimb development

doi: 10.1016/j.isci.2024.108838

Figure Lengend Snippet:

Article Snippet: Rat monoclonal perlecan (HSPG2; clone A7L6) , Santa Cruz Biotechnology , Cat# sc-33707; RRID: AB_627714.

Techniques: Recombinant, Protease Inhibitor, Plasmid Preparation, Protein Extraction, Hydroxyproline Assay, Mass Spectrometry, Software, Microscopy